Once more thank you to Patrick Trieu-Cuot for his suggestions and encouragements.
New function of primer design: AmplifX
searches for pairs of primers with no, or one or the other of both primers fixed. (Algorithm in the course of improvement, welcome experience feedback)
- Print and export of the chart.
Support of DNA Strider
files as target sequence (of which a new version for Mac OS X is available)
New information on the amplified sequence: addition of a suggested annealing temperature for the PCR. (According to Rychlik.W et al. in NAR (1990), vol. 18 pp 6409-6412)
Possibility of searching sub-sequences in the target sequence (access by the menus already existing employees yet for search in the primer list)
Use of styled text for the copied amplified sequence: useful to quickly locate the primers, when the sequence is pasted in an application which support styles
Corrections and improvements
Adequacy of the use of the relation "Superior or equal to the cut off" for the drawing and the search for matches
the use of right and left arrows, and the suppress key work in the « numeric-range » fields of the preferences’s window
Correction of an error occuring while importing GeneBank format files.
Correction of the display of information of the current selection of the sequence target at the time of the use of the menu "SelectAll", "lowercases", "uppercases" and while un-selecting
Maintenance of the lower/uppercases features on the complementary strands
Best updating of the “ default file “ radio-button state after a “ save as “ operation.
When the window of search is displayed, the focus one is put into the field of search to avoid typing into the target sequence.
Correction of a bug related to the list of the recent files preserved in the preference file and which could involve in certain circumstances a crash at AmplifX starting and whose only solution was yet to delete the preference file.
- Correction of a bug that could occur if the quality scores had to be re-computed at the automatic loading of the primer’s default file.
Correction of the placement of reverse primers over the chart (for small amplified fragments the primer length is not negligeable)